Journal: Transplantation Direct

Article Title: Subnormothermic Ex Vivo Porcine Kidney Perfusion Improves Energy Metabolism: Analysis Using 31 P Magnetic Resonance Spectroscopic Imaging

doi: 10.1097/TXD.0000000000001354

Figure Lengend Snippet: Ex vivo subnormothermic perfusion improves kidney perfusion. A, Experimental groups and design. Kidneys were retrieved after 60 min warm ischemia and placed into a hypothermic + passive oxygenation (4 °C), hypothermic + active oxygenation (4 °C + O 2 ), or subnormothermic + active oxygenation (22 °C + O 2 ) perfusion machine with or without oxygen for 4 h. Kidney were autografted into the same pig. Dynamic-contrast enhanced MRI analysis was performed prior (4 °C + O 2 , 22 °C + O 2 ) and after transplantation (4 °C, 4 °C + O 2 , 22 °C + O 2 ). B, Pretransplant representative Gd uptake in cortex (top) and medulla (bottom) of kidneys during hypothermic (blue line) or subnormothermic (red) perfusion and Gd perfusion-DS quantification (right, n = 5/group). Bars indicate mean ± SEM, and asterisks indicate the significance of the difference between perfusions methods by Student’s t test or 1-way ANOVA and Tukey test. * P < 0.05, ** P < 0.01. n = 4 to 6 per group. DS, descending slope.

Article Snippet: DCD grafts are particularly vulnerable to ischemia-reperfusion (IR) injury, an issue that is not addressed at all by current standard storage strategies, including static cold storage and nonoxygenated hypothermic machine perfusion (eg, LifePort)., With this approach, prolonged periods (>24 h) of cold ischemia are associated with tubular necrosis, DGF, and poor graft survival., , Although static cold storage is the most prevalent method for renal allograft preservation, hypothermic machine perfusion without oxygen was shown to reduce DGF and to improve 1- and 3-y graft survival.

Techniques: Ex Vivo, Transplantation Assay

Journal: International Journal of Molecular Sciences

Article Title: Mitoquinone Alleviates Donation after Cardiac Death Kidney Injury during Hypothermic Machine Perfusion in Rat Model

doi: 10.3390/ijms241914772

Figure Lengend Snippet: Scheme of the kidney preparation and perfusion. Right kidney was excised, flashed with pure buffer, and perfused for 22 h with or without MitoQ in the hypothermic condition (4 °C) with controlled pressure (50–120 mmHg).

Article Snippet: The right kidney was immediately excised, cannulated by the renal artery, and connected to the hypothermic machine perfusion system (Single Channel Perfusion System, EMKA Technologies, Paris, France) (DBD group) or tied for 30 min with controlled temperature (37 °C) to mimic DCD graft donation and subsequently excised, cannulated by the renal artery, and connected to the hypothermic machine perfusion system (experimental groups: DCD and DCD + MitoQ) ( ).

Techniques: